《植物生理学报》 2010, 46(1): 24-28

NaCl 胁迫下外源 NO 供体硝普钠(SNP)对盐地碱蓬种子萌发的影响

袁芳, 杨剑超, 陈敏, 宋杰, 隋娜, 王宝山*

山东师范大学生命科学学院逆境植物重点实验室, 济南 250014

通信作者：王宝山；E-mail: bswang@sdnu.edu.cn；Tel: 0531-86180 197

摘　要：

用添加与不添加0.1 mmol•L-1 NO供体硝普钠(SNP)的800 mmol•L-1 NaCl溶液处理盐地碱蓬种子后, 800 mmol•L-1 NaCl 处理下盐地碱蓬种子的萌发率、含水量和吸水速率显著增加, 胚中脯氨酸的含量降低, 但对 Na+、K+ 和可溶性糖含量无显 著影响。表明 0.1 mmol•L-1 SNP 缓解 800 mmol•L-1 NaCl 对盐地碱蓬种子萌发抑制的主要原因是盐地碱蓬种子含水量的提 高, 从而缓解了盐的渗透胁迫。

关键词：盐地碱蓬; NaCl胁迫; SNP; 种子萌发; 渗透胁迫

收稿：2009-10-15   修定：2009-12-28

资助：国家自然科学基金(30670177)和国家海洋“863” 重点项目(2007AA091701)。

Effect of NO Donor Sodium Nitroprusside (SNP) on Seed Germination of Suaeda salsa L. under NaCl Stress

YUAN Fang, YANG Jian-Chao, CHEN Min, SONG Jie, SUI Na, WANG Bao-Shan*

Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, China

Corresponding author: WANG Bao-Shan; E-mail: bswang@sdnu.edu.cn; Tel: 0531-86180 197

Abstract:

The effect of NO donor sodium nitroprusside (SNP) on seed germination physiology under NaClstress was examined using the seeds of Suaeda salsa treated with NaCl (800 mmol·L^-1) containing 0.1 mmol·L^-1 SNP or not. Results were as follows, seed germination rate of S. salsa decreased remarkably when the seeds were treated with 800 mmol·L^-1 NaCl, while 0.1 mmol·L^-1 SNP significantly alleviated the germination inhibition induced by NaCl. Water content and water absorption rate of seeds treated with SNP were higher than those of the control, while the proline content of embryos was lower. SNP treatment produced no significant influence on Na⁺, K⁺ and soluble carbohydrate content of the embryos under NaCl stress. The results suggested that the main reason for SNP alleviation of NaCl stress was that it could significantly increase the water content of seeds and alleviate osmotic stress generated by NaCl.

Key words: Suaeda salsa; NaCl stress; SNP; seed germination; osmotic stress